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human mesenchymal stem cell culture and transformation mscs  (Lonza)
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Lonza human mesenchymal stem cell culture and transformation mscs
(A) Phase contrast images of <t>MSCs,</t> before (left) or after (right) the oncogenic NiSO4 treatment. (B) Proliferation of NiSO4-treated MSCs and controls measured using BrdU labeling after 3 days in culture. (C) Expression of the cell surface markers CD44, CD133, and Stro-1 quantified by flow cytometry in untreated and NiSO4-treated MSCs. (D) Expression of topoisomerase II-α, measured by immunostaining in MSCs and NiSO4-treated MSCs after 3 days in culture. (E) Telomerase (hTR) expression detected by FISH either 3 or 11 days after ending the NiSO4 treatment, as well as in untreated MSCs and in transformed MSCs (tMSCs). Fluorescence signal intensities are quantified in the bar graph and representative images are shown. (F) High-content analysis of nuclear descriptors derived from MSCs, NiSO4-treated MSCs (3 days after treatment), and tMSCs. Classification is visualized in 3D space after principal component analysis as well as with the parsing index. (G) Transformation indexes for MSC <t>cells</t> <t>cultured</t> on different biomaterial substrates (listed in the table) after NiSO4 exposure. The indexes represent hTR expression relative to untransformed MSCs (value 0) and tMSCs (value 1). hTR analysis was performed 11 days after ending the oncogenic treatment. (H) Highcontent imaging parsing indexes plotted against Transformation Indexes for MSCs cultured on different substrates as in (G). R, Pearson correlation coefficient. *, P < 0.01.
Human Mesenchymal Stem Cell Culture And Transformation Mscs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Phase contrast images of MSCs, before (left) or after (right) the oncogenic NiSO4 treatment. (B) Proliferation of NiSO4-treated MSCs and controls measured using BrdU labeling after 3 days in culture. (C) Expression of the cell surface markers CD44, CD133, and Stro-1 quantified by flow cytometry in untreated and NiSO4-treated MSCs. (D) Expression of topoisomerase II-α, measured by immunostaining in MSCs and NiSO4-treated MSCs after 3 days in culture. (E) Telomerase (hTR) expression detected by FISH either 3 or 11 days after ending the NiSO4 treatment, as well as in untreated MSCs and in transformed MSCs (tMSCs). Fluorescence signal intensities are quantified in the bar graph and representative images are shown. (F) High-content analysis of nuclear descriptors derived from MSCs, NiSO4-treated MSCs (3 days after treatment), and tMSCs. Classification is visualized in 3D space after principal component analysis as well as with the parsing index. (G) Transformation indexes for MSC cells cultured on different biomaterial substrates (listed in the table) after NiSO4 exposure. The indexes represent hTR expression relative to untransformed MSCs (value 0) and tMSCs (value 1). hTR analysis was performed 11 days after ending the oncogenic treatment. (H) Highcontent imaging parsing indexes plotted against Transformation Indexes for MSCs cultured on different substrates as in (G). R, Pearson correlation coefficient. *, P < 0.01.

Journal: Experimental cell research

Article Title: High-content image informatics of the structural nuclear protein NuMA parses trajectories for stem/progenitor cell lineages and oncogenic transformation

doi: 10.1016/j.yexcr.2016.12.018

Figure Lengend Snippet: (A) Phase contrast images of MSCs, before (left) or after (right) the oncogenic NiSO4 treatment. (B) Proliferation of NiSO4-treated MSCs and controls measured using BrdU labeling after 3 days in culture. (C) Expression of the cell surface markers CD44, CD133, and Stro-1 quantified by flow cytometry in untreated and NiSO4-treated MSCs. (D) Expression of topoisomerase II-α, measured by immunostaining in MSCs and NiSO4-treated MSCs after 3 days in culture. (E) Telomerase (hTR) expression detected by FISH either 3 or 11 days after ending the NiSO4 treatment, as well as in untreated MSCs and in transformed MSCs (tMSCs). Fluorescence signal intensities are quantified in the bar graph and representative images are shown. (F) High-content analysis of nuclear descriptors derived from MSCs, NiSO4-treated MSCs (3 days after treatment), and tMSCs. Classification is visualized in 3D space after principal component analysis as well as with the parsing index. (G) Transformation indexes for MSC cells cultured on different biomaterial substrates (listed in the table) after NiSO4 exposure. The indexes represent hTR expression relative to untransformed MSCs (value 0) and tMSCs (value 1). hTR analysis was performed 11 days after ending the oncogenic treatment. (H) Highcontent imaging parsing indexes plotted against Transformation Indexes for MSCs cultured on different substrates as in (G). R, Pearson correlation coefficient. *, P < 0.01.

Article Snippet: Human mesenchymal stem cell culture and transformation MSCs were obtained from Lonza and cultured according to the supplier’s recommendations and reagents.

Techniques: Labeling, Expressing, Flow Cytometry, Immunostaining, Transformation Assay, Fluorescence, High Content Screening, Derivative Assay, Cell Culture, Imaging